|Application ||WB, ICC, E|
|Description||ApoM (apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment. Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.|
|Immunogen||Purified recombinant fragment of ApoM expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Other Names||Apolipoprotein M, Apo-M, ApoM, Protein G3a, APOM, G3A, NG20|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ApoM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.|
|Cellular Location||Secreted Note=Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins (LDL)|
|Tissue Location||Plasma protein. Expressed in liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
1. Xu,N. & Dahlback,B.1999. J. Biol. Chem. 274 (44):31286-31290. 2. Christian Wolfrum, Matthew N Poy & Markus Stoffel. 2005. Nat Med. 11(4):418-22. 3. Xu,N., Nilsson-Ehle,P. & Ahren,B. 2004. J. Nutr. Biochem. 15 (10):579-582. 4. Zhang,X.Y. ,et al.2004. Acta Histochem. 106 (2):123-128.
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