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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD31 Antibody   

CD31 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CD31 Antibody AO1104a
    Figure 1: Western blot analysis using CD31 mouse mAb against truncated CD31 recombinant protein.
  • IHC - CD31 Antibody AO1104a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), lymphonodus tissue (B) and breast cancer (C), showing cytoplasmic localization of vascular endothelial cells using CD31 mouse mAb with DAB staining.
  • IHC - CD31 Antibody AO1104a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human placenta using CD31 mouse mAb.
  • IF - CD31 Antibody AO1104a
    Figure 4:Immunofluorescence analysis of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16284
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F7B2
Isotype IgG1
Calculated MW 82536 Da
Description CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Immunogen Purified recombinant fragment of human CD31 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 5175
Other Names Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD31 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Cellular Location Isoform Long: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells
Tissue Location Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).
Research Areas
Citations (0)

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1. Mayr U et al. Circ Res 98:412-20 (2006). 2. Bingle L et al. Br J Cancer 94:101-7 (2006). 3. Wynne F et al. Reproduction 131:721-32 (2006).

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$ 385.00
Cat# AO1104a
(40 western blots)
Availability: 7-10 days
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