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>   home   >   Products   >   Primary Antibodies   >   Cytokeratin (Pan) Antibody   

Cytokeratin (Pan) Antibody

Purified Mouse Monoclonal Antibody

  • WB - Cytokeratin (Pan) Antibody AO1116a
    Figure 1: Western blot analysis using CK mouse mAb against truncated CK5 recombinant protein.
  • IHC - Cytokeratin (Pan) Antibody AO1116a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma?, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.
  • IHC - Cytokeratin (Pan) Antibody AO1116a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human Skin tissues using CK mouse mAb
  • IF - Cytokeratin (Pan) Antibody AO1116a
    Figure 4: Immunofluorescence staining of methanol-fixed Eca-109 (left) and HepG2 (right) cells showing cytoplasmic localization.
  • FC - Cytokeratin (Pan) Antibody AO1116a
    Figure 4: Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .
  •  - Cytokeratin (Pan) Antibody AO1116a
    Figure 3: Immunocytochemistry analysis of HCC827 cells, untransfected(left) or transfected with GFP(right) using anti-GFP monoclonal antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13647
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7H8C4
Isotype IgG1
Calculated MW 62378 Da
Description Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Immunogen Purified recombinant fragment of CK5 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3852
Other Names Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, KRT5
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin (Pan) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT5
Citations (0)

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1. Scope A. Schwendenwein I. Frommlet F. Vet Rec. 2006, Dec 16, 159(25): 839-43. 2. Somjen D. Katzburg S. Posner GH. et al. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.

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$ 325.00
Cat# AO1116a
(40 western blots)
Availability: 7-10 days
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