|Application ||WB, IHC, FC, E|
|Description||GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.|
|Immunogen||Purified recombinant fragment of GFP expressed in E. Coli.|
|Formulation||Purified antibody in PBS containing 0.03% sodium azide.|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. 2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. 3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68.
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