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ETV4 Antibody

Purified Mouse Monoclonal Antibody

  • WB - ETV4 Antibody AO1193a
    Figure 1: Western blot analysis using ETV4 mouse mAb against K562 cell lysate (1).
  • WB - ETV4 Antibody AO1193a
    Figure 2: Western blot analysis using ETV4 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ETV4 cDNA (2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P43268
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1A2G3
Isotype IgG1
Calculated MW 54kDa
Description ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-α. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.
Immunogen Purified recombinant fragment of human ETV4 (aa50-109) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2118
Other Names ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3, ETV4, E1AF, PEA3
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETV4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETV4
Synonyms E1AF, PEA3
Function Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
Cellular Location Nucleus.
Research Areas
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1. J Biol Chem. 2005 Apr 1;280(13):12503-16. 2. Mol Carcinog. 2006 Sep;45(9):667-75. 3. Oncol Rep. 2006 Jul;16(1):153-8.

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$ 385.00
Cat# AO1193a
(40 western blots)
Availability: 7-10 days
Bulk Size
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