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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   F8 Antibody   

F8 Antibody

Purified Mouse Monoclonal Antibody

  • WB - F8 Antibody AO1228a
    Figure 1: Western blot analysis using F8 mouse mAb against truncated Trx-F8 recombinant protein (1).
  • IHC - F8 Antibody AO1228a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using BHMT mouse mAb
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00451
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5E9B2
Isotype IgG1
Calculated MW 267009 Da
Description F8: coagulation factor VIII, procoagulant component. This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
Immunogen Purified recombinant fragment of F8 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2157
Other Names Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component, Factor VIIIa heavy chain, 200 kDa isoform, Factor VIIIa heavy chain, 92 kDa isoform, Factor VIII B chain, Factor VIIIa light chain, F8, F8C
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsF8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name F8
Synonyms F8C
Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Cellular Location Secreted, extracellular space.
Research Areas
Citations (0)

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1. Thromb Haemost. 2007 Nov;98(5):1031-9. 2. Blood. 2008 Apr 1;111(7):3468-78.

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$ 325.00
Cat# AO1228a
(40 western blots)
Availability: 7-10 days
Bulk Size
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