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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EPHB4 Antibody   

EPHB4 Antibody

Purified Mouse Monoclonal Antibody

  • WB - EPHB4 Antibody AO1275a
    Figure 1: Western blot analysis using EphB4 mouse mAb against extracellular domain of human EphB4 (aa16-539).
  • IF - EPHB4 Antibody AO1275a
    Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • IHC - EPHB4 Antibody AO1275a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Heart tissues using MYL3 mouse mAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54760
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B5
Isotype IgG1
Calculated MW 108kDa
Description EphB4: EPH receptor B4, also known as Htk, Myk1, Tyro11. Entrez Protein: NP_004435. It is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EphB4 protein binds to ephrin-B2 and plays an essential role in vascular development.
Immunogen Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2050
Other Names Ephrin type-B receptor 4,, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11, EPHB4, HTK, MYK1, TYRO11
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPHB4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPHB4
Synonyms HTK, MYK1, TYRO11
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix
Research Areas
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1. Br J Cancer. 2007 Apr 10;96(7):1083-91. 2. Dig Liver Dis. 2007 Aug;39(8):725-32.

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$ 325.00
Cat# AO1275a
(40 western blots)
Availability: 7-10 days
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