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CD80 Antibody

Purified Mouse Monoclonal Antibody

  • IHC - CD80 Antibody AO1281a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-CD80 mAb
  • IF - CD80 Antibody AO1281a
    Figure 2: Confocal immunofluorescence analysis of BCBL-1 cells using anti-CD80 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • WB - CD80 Antibody AO1281a
    Figure 1: Western blot analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P33681
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A2
Isotype IgG1
Calculated MW 55kDa
Description The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. Its principal mode of action is by binding to CD28. Along with CD86, these molecules provide the necessary stimuli to prime T cells against antigens presented by antigen-presenting cells. CD80 and CD86 also bind to CTLA-4, a cell surface molecule expressed on activated T cells. Interactions between CD80 or CD86 with CTLA-4 decrease the response of T cells. Mouse research by scientists at Emory University showed that estrogen-related bone loss is linked to recently discovered pathways involving various proteins, such as CD80 and other functions. In a nutshell, reactive oxygen stimulates dendritic cells, which activate other immune cells to up-regulate production of CD80, the molecule co-responsible for T cell activation."When this pathway is activated, it leads to increased T cell TNF production and ultimately to bone loss."In turn, T cells produce a protein, Tumor Necrosis Factor, which increases the formation of osteoclasts in rodents and humans. Osteoclasts cause minerals to be released from the bone, so that calcium is taken into the bloodstream to be used for other functions of the body. Osteoclast differentiation is inhibited by osteoprotegerin; Estrogen stimulates osteoprotegerin production.
Immunogen Purified recombinant fragment of CD80 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 941
Other Names T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, CD80, CD80, CD28LG, CD28LG1, LAB7
Dilution IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD80 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD80
Synonyms CD28LG, CD28LG1, LAB7
Function Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed on activated B-cells, macrophages and dendritic cells
Research Areas
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1. Transplant Proc. 2008 Oct;40(8):2729-33. 2. Nat Med. 2007 Dec;13(12):1440-9.

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$ 325.00
Cat# AO1281a
Availability: 7-10 days
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