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PAK2 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E |
|---|---|
| Primary Accession | Q13177 |
| Reactivity | Human, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 3B5 |
| Isotype | IgG1 |
| Calculated MW | 61kDa |
| Description | PAK2, also known as P21 (CDKN1A)-activated kinase 2. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. PAK2 has been shown to interact with SH3KBP1, CDC42 and Abl gene. |
| Immunogen | Purified recombinant fragment of PAK2 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 5062 |
|---|---|
| Other Names | Serine/threonine-protein kinase PAK 2, 2.7.11.1, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK-2p27, p27, PAK-2p34, p34, C-t-PAK2, PAK2 |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 IF~~1/200 - 1/1000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PAK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PAK2 |
|---|---|
| Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed:7744004, PubMed:19273597, PubMed:19923322, PubMed:9171063, PubMed:12853446, PubMed:16617111, PubMed:33693784). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed:7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed:7744004). Full- length PAK2 stimulates cell survival and cell growth (PubMed:7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed:21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed:21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed:21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed:21555521, PubMed:27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed:19273597, PubMed:19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed:9171063, PubMed:12853446, PubMed:16617111). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed:15234964). |
| Cellular Location | [Serine/threonine-protein kinase PAK 2]: Cytoplasm Nucleus Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane |
| Tissue Location | Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. J Immunol. 2004 Jun 15;172(12):7324-34. 2. J Mol Biol. 2007 Jul 20;370(4):620-32.
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$ 385.00
Cat# AO1296a
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