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NT5E Antibody

Purified Mouse Monoclonal Antibody

  • IHC - NT5E Antibody AO1306a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), cholangiocarcinorna (B), lymph node (C) and esophagus (D) tissues using NT5E mouse mAb with DAB staining.
  • WB - NT5E Antibody AO1306a
    Figure 1: Western blot analysis using FRK mouse mAb against K562 cell lysate (1).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21589
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1D7
Isotype IgG1
Calculated MW 70kDa
Description 5'-nucleotidase, ecto (NT5E), also known as CD73 (Cluster of Differentiation 73). Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
Immunogen Purified recombinant fragment of NT5E expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4907
Other Names 5'-nucleotidase, 5'-NT,, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE
Dilution IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNT5E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NT5E
Synonyms NT5, NTE
Function Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Research Areas
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1. Oncol Rep. 2007 Jun;17(6):1341-6. 2. Neurochem Int. 2003 Dec;43(7):621-8.

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$ 325.00
Cat# AO1306a
(40 western blots)
Availability: 7-10 days
Bulk Size
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