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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   WNT1 Antibody   

WNT1 Antibody

Purified Mouse Monoclonal Antibody

     
  • IF - WNT1 Antibody AO1313a
    Figure3: Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • WB - WNT1 Antibody AO1313a
    Figure 1: Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
  • IHC - WNT1 Antibody AO1313a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb
  • FC - WNT1 Antibody AO1313a
    Figure 4: Flow cytometric analysis of Hela cells using WNT1 mouse mAb (green) and negative control (purple).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession P04628
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 10C8
Isotype IgG1
Calculated MW 41kDa
Description WNT1: wingless-type MMTV integration site family, member 1. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant rolein Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Immunogen Purified recombinant fragment of WNT1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 7471
Other Names Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, WNT1, INT1
Dilution IF~~1/200 - 1/1000
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT1
Synonyms INT1
Function Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.
Cellular Location Secreted, extracellular space, extracellular matrix
Research Areas
Citations (0)

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References

1. Blood. 2008 Jan 1;111(1):122-31. 2. BMC Cancer. 2005 May 24;5:53.

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$ 325.00
Cat# AO1313a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
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