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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NKX2.5 Antibody   

NKX2.5 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - NKX2.5 Antibody AO1319a
    Figure 1: Western blot analysis using NKX2.5 mouse mAb against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1).
  • FC - NKX2.5 Antibody AO1319a
    Figure 4: Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
  • ICC - NKX2.5 Antibody AO1319a
    Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IF - NKX2.5 Antibody AO1319a
    Figure 2: Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IF - NKX2.5 Antibody AO1319a
    Figure 3: Confocal immunofluorescence analysis of 3T3-L1 cells using anti-JAK3 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P52952
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E1
Isotype IgG1
Calculated MW 35kDa
Description NKX2.5: NK2 transcription factor related, locus 5 (Drosophila), also known as CSX. It is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
Immunogen Purified recombinant fragment of human NKX2.5 expressed in E. Coli.
Formulation Antibody are purified by protein G affinity chromatography.
Liquid in PBS containing 0.03% sodium azide.
Additional Information
Gene ID 1482
Other Names Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, NKX2-5, CSX, NKX2.5, NKX2E
Dilution WB~~1/500 - 1/2000
FC~~1:200~~400
ICC~~1:200~~1000
IF~~1:200~1000.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX2.5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX2-5
Synonyms CSX, NKX2.5, NKX2E
Function Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Cellular Location Nucleus.
Tissue Location Expressed only in the heart.
Research Areas
Citations (0)

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References

1. Stem Cells Dev. 2005 Aug;14(4):425-39. 2. Cancer Res. 2003 Sep 1;63(17):5329-34. 3. Circ J. 2002 Jun;66(6):561-3.

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$ 325.00
Cat# AO1319a
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