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WNT10B Antibody

Purified Mouse Monoclonal Antibody

  • WB - WNT10B Antibody AO1325a
    Figure 1: Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).
  • IHC - WNT10B Antibody AO1325a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using WNT10B mouse mAb with DAB staining.
  • IF - WNT10B Antibody AO1325a
    Figure 3: Confocal immunofluorescence analysis of PANC-1 cells using WNT10B mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • FC - WNT10B Antibody AO1325a
    Figure 4: Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00744
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5A7
Isotype IgG1
Calculated MW 43kDa
Description WNT10B: wingless-type MMTV integration site family, member 10B. The WNT family consists of structurally related secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is ikely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10B protein at the amino acid level. The WNT10B gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
Immunogen Purified recombinant fragment of human WNT10B expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 7480
Other Names Protein Wnt-10b, Protein Wnt-12, WNT10B, WNT12
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT10B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT10B
Synonyms WNT12
Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain
Research Areas
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1. Oncogene. 1997 Mar 13;14(10):1249-53. 2. Int J Oncol. 2001 Dec;19(6):1187-92

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$ 385.00
Cat# AO1325a
(40 western blots)
Availability: 7-10 days
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