|Application ||WB, IHC, E|
|Description||Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.|
|Immunogen||Purified recombinant fragment of human KLHL21 expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Kelch-like protein 21, KLHL21, KIAA0469|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLHL21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.|
|Cellular Location||Cytoplasm, cytoskeleton, spindle. Note=Localizes to the spindle midzone and targets CUL3 to this region|
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1. DNA Res. 1997 Oct 31;4(5):345-9. 2. DNA Res. 2005;12(2):117-26. 3. J Cell Biol. 2009 Dec 14;187(6):791-800.
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