|Application ||WB, IHC, E|
|Description||ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). ALDH1A1 protein is a known mesencephalic dopaminergic marker. ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid .ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea .ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development .The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells.|
|Immunogen||Purified recombinant fragment of human ALDH1A1 expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Retinal dehydrogenase 1, RALDH 1, RalDH1, 22.214.171.124, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALDH1A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ALDC, ALDH1, PUMB1|
|Function||Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).|
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1. J Hum Genet. 2009 Jun;54(6):317-23. 2. J Hum Genet. 2009 Oct;54(10):564-71. 3. J Neurosci Res. 2010 Feb 15;88(3):686-94.
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