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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   WIF1 Antibody   

WIF1 Antibody

Purified Mouse Monoclonal Antibody

  • WB - WIF1 Antibody AO1463a
    Figure 1: Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
  • IHC - WIF1 Antibody AO1463a
    Figure 2: Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.
  • IF - WIF1 Antibody AO1463a
    Figure 3: Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y5W5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1G5
Isotype IgG1
Calculated MW 42kDa
Description The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Immunogen Purified recombinant fragment of human WIF1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 11197
Other Names Wnt inhibitory factor 1, WIF-1, WIF1
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWIF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WIF1
Function Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
Cellular Location Secreted.
Research Areas
Citations (0)

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1. BMC Cancer. 2009 Jul 1;9:217. 2. Cancer Res. 2009 Nov 15;69(22):8603-10.

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$ 325.00
Cat# AO1463a
(40 western blots)
Availability: 7-10 days
Bulk Size
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