|Application ||WB, FC, ICC, E|
|Description||This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.|
|Immunogen||Synthesized peptide derived from internal of human Oct4.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Oct4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||OCT3, OCT4, OTF3|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.|
|Cellular Location||Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern|
|Tissue Location||Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.|
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Provided below are standard protocols that you may find useful for product applications.
1. Stem Cells. 2010 May;28(5):885-93. 2. Mol Med. 2010 Jul-Aug;16(7-8):247-53. 3. Med Sci (Paris). 2010 Apr;26(4):411-6.
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