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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CCNB1 Antibody   

CCNB1 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CCNB1 Antibody AO1476a
    Figure 1: Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
  • IF - CCNB1 Antibody AO1476a
    Figure 2: Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - CCNB1 Antibody AO1476a
    Figure 3: Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14635
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 5G6
Isotype IgG1
Calculated MW 60kDa
Description The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .
Immunogen Purified recombinant fragment of human CCNB1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 891
Other Names G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCNB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNB1
Synonyms CCNB
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas
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1. Br J Cancer. 2009 Oct 20;101(8):1461-8. 2. Cancer Res. 2010 Feb 1;70(3):1265-74. 3. J Biol Chem. 2010 Jun 4;285(23):17833-45.

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$ 325.00
Cat# AO1476a
(40 western blots)
Availability: 7-10 days
Bulk Size
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