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ACTA2 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ACTA2 Antibody AO1514a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

    detail
  • WB - ACTA2 Antibody AO1514a
    Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.
    detail
  • IF - ACTA2 Antibody AO1514a
    Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
    detail
  • FC - ACTA2 Antibody AO1514a
    Figure 3: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession P62736
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 1H8
Isotype IgG1
Calculated MW 42kDa
Description Actin,alpha 2, smooth muscle, aorta, major constituent of thin filaments.
Immunogen Purified recombinant fragment of human ACTA2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 59
Other Names Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTA2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTA2
Synonyms ACTSA, ACTVS
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)
citation

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References

1. Nat Genet. 2007 Dec;39(12):1488-93. 2. Virchows Arch. 2007 Dec;451(6):999-1007.

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$ 385.00
Cat# AO1514a
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