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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EIF4E Antibody   

EIF4E Antibody

Purified Mouse Monoclonal Antibody

  • E - EIF4E Antibody AO1540a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - EIF4E Antibody AO1540a
    Figure 1: Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
  • IHC - EIF4E Antibody AO1540a
    Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.
  • IHC - EIF4E Antibody AO1540a
    Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using EIF4E mouse mAb with DAB staining.
  • IF - EIF4E Antibody AO1540a
    Figure 4: Immunofluorescence analysis of GC-7901 cells using EIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - EIF4E Antibody AO1540a
    Figure 5: Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06730
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5D11
Isotype IgG1
Calculated MW 25kDa
Description eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Immunogen Purified recombinant fragment of human EIF4E expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 1977
Other Names Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4E Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Research Areas
Citations (0)

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1. Ann Surg Oncol. 2008 Nov;15(11):3207-15. 2. J Biol Chem. 2008 Sep 12;283(37):25227-37.

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$ 385.00
Cat# AO1540a
(40 western blots)
Availability: 7-10 days
Bulk Size
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