|Application ||WB, IHC, ICC, E|
|Description||This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. (provided by RefSeq)|
|Immunogen||Purified recombinant fragment of human EEF2 expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Elongation factor 2, EF-2, EEF2, EF2|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EEF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.|
|Cellular Location||Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.|
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Provided below are standard protocols that you may find useful for product applications.
1. Mol Cell Biol. 2008 Dec;28(23):7050-65. 2. Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R326-33.
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