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ApoB Antibody

Purified Mouse Monoclonal Antibody

  • E - ApoB Antibody AO1564a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - ApoB Antibody AO1564a
    Figure 1: Western blot analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)
  • IF - ApoB Antibody AO1564a
    Figure 2: Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - ApoB Antibody AO1564a
    Figure 3: Flow cytometric analysis of serun using ApoB mouse mAb (green) and negative control (purple).
  • FC - ApoB Antibody AO1564a
    Figure 3: Flow cytometric analysis of PBMC using ApoB mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04114
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6G6
Isotype IgG1
Calculated MW 516kDa
Description This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
Immunogen Purified recombinant fragment of human ApoB expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 338
Other Names Apolipoprotein B-100, Apo B-100, Apolipoprotein B-48, Apo B-48, APOB
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsApoB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Cellular Location Cytoplasm. Secreted
Research Areas
Citations (0)

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1. Cell Host Microbe. 2008 Dec 11;4(6):555-66. 2. Atherosclerosis. 2009 Sep;206(1):17-30.

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$ 385.00
Cat# AO1564a
(40 western blots)
Availability: 7-10 days
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