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PINCH Antibody

Purified Mouse Monoclonal Antibody

  • E - PINCH Antibody AO1591a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - PINCH Antibody AO1591a
    Figure 1: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
  • WB - PINCH Antibody AO1591a
    Figure 2: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
  • IF - PINCH Antibody AO1591a
    Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - PINCH Antibody AO1591a
    Figure 4: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48059
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5G7
Isotype IgG1
Calculated MW 37kDa
Description The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human PINCH expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3987
Other Names LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH-1, Renal carcinoma antigen NY-REN-48, LIMS1, PINCH, PINCH1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPINCH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIMS1
Synonyms PINCH, PINCH1
Function Adapter protein in a cytoplasmic complex linking beta- integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in most tissues except in the brain.
Research Areas
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1. J Biol Chem. 2009 Feb 27;284(9):5836-44. 2. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20677-82.

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$ 385.00
Cat# AO1591a
(40 western blots)
Availability: 7-10 days
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