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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   PINCH Antibody   

PINCH Antibody

Purified Mouse Monoclonal Antibody

     
  • E - PINCH Antibody AO1591a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - PINCH Antibody AO1591a
    Figure 1: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) recombinant protein. (Expected MW is 44.2 kDa)
  • WB - PINCH Antibody AO1591a
    Figure 2: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.
  • IF - PINCH Antibody AO1591a
    Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - PINCH Antibody AO1591a
    Figure 4: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC, E
Primary Accession P48059
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5G7
Isotype IgG1
Calculated MW 37kDa
Description The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human PINCH expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Other Names LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH-1, Renal carcinoma antigen NY-REN-48, LIMS1, PINCH, PINCH1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPINCH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIMS1
Synonyms PINCH, PINCH1
Function Adapter protein in a cytoplasmic complex linking beta- integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in most tissues except in the brain. EMBL; U09284; AAA20086.2; -; mRNA EMBL; AK296992; BAH12469.1; -; mRNA EMBL; AK302411; BAH13699.1; -; mRNA EMBL; AK304260; BAH14143.1; -; mRNA EMBL; AK314217; BAG36891.1; -; mRNA EMBL; AC010095; AAY14983.1; -; Genomic_DNA EMBL; AC012487; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC005341; AAH05341.1; -; mRNA CCDS; CCDS2078.1; -. [P48059-1] CCDS; CCDS54382.1; -. [P48059-2] CCDS; CCDS54383.1; -. [P48059-4] CCDS; CCDS54384.1; -. [P48059-5] CCDS; CCDS54385.1; -. [P48059-3] PIR; JC2324; JC2324 RefSeq; NP_001180411.1; NM_001193482.1. [P48059-4] RefSeq; NP_001180412.1; NM_001193483.2. [P48059-2] RefSeq; NP_001180413.1; NM_001193484.1. [P48059-5] RefSeq; NP_001180414.1; NM_001193485.2. [P48059-3] RefSeq; NP_001180417.1; NM_001193488.1. [P48059-1] RefSeq; NP_004978.2; NM_004987.5. [P48059-1] UniGene; Hs.597715; - UniGene; Hs.613268; - PDB; 1G47; NMR; -; A=1-70 PDB; 1NYP; NMR; -; A=188-251 PDB; 1U5S; NMR; -; B=188-251 PDB; 2COR; NMR; -; A=125-190 PDB; 2D8X; NMR; -; A=71-127 PDB; 2KBX; NMR; -; B=1-70 PDB; 3F6Q; X-ray; 1.60 A; B=6-68 PDB; 4HI8; X-ray; 1.20 A; B=6-68 PDB; 4HI9; X-ray; 1.20 A; B=6-68 PDBsum; 1G47; - PDBsum; 1NYP; - PDBsum; 1U5S; - PDBsum; 2COR; - PDBsum; 2D8X; - PDBsum; 2KBX; - PDBsum; 3F6Q; - PDBsum; 4HI8; - PDBsum; 4HI9; - ProteinModelPortal; P48059; - SMR; P48059; - BioGrid; 110175; 37 DIP; DIP-40671N; - IntAct; P48059; 52 MINT; P48059; - STRING; 9606.ENSP00000446121; - iPTMnet; P48059; - PhosphoSitePlus; P48059; - BioMuta; LIMS1; - DMDM; 18266876; - OGP; P48059; - EPD; P48059; - MaxQB; P48059; - PaxDb; P48059; - PeptideAtlas; P48059; - PRIDE; P48059; - ProteomicsDB; 55846; - ProteomicsDB; 55847; -. [P48059-2] ProteomicsDB; 55848; -. [P48059-3] ProteomicsDB; 55849; -. [P48059-4] ProteomicsDB; 55850; -. [P48059-5] DNASU; 3987; - Ensembl; ENST00000332345; ENSP00000331775; ENSG00000169756. [P48059-1] Ensembl; ENST00000338045; ENSP00000337598; ENSG00000169756. [P48059-3] Ensembl; ENST00000393310; ENSP00000376987; ENSG00000169756. [P48059-1] Ensembl; ENST00000409441; ENSP00000387264; ENSG00000169756. [P48059-5] Ensembl; ENST00000410093; ENSP00000386926; ENSG00000169756. [P48059-4] Ensembl; ENST00000544547; ENSP00000437912; ENSG00000169756. [P48059-2] GeneID; 3987; - KEGG; hsa:3987; - UCSC; uc002teg.4; human. [P48059-1] CTD; 3987; - DisGeNET; 3987; - EuPathDB; HostDB:ENSG00000169756.16; - GeneCards; LIMS1; - HGNC; HGNC:6616; LIMS1 HPA; HPA058455; - HPA; HPA061230; - MIM; 602567; gene neXtProt; NX_P48059; - OpenTargets; ENSG00000169756; - PharmGKB; PA30389; - eggNOG; KOG2272; Eukaryota eggNOG; ENOG410XP46; LUCA GeneTree; ENSGT00760000118910; - HOGENOM; HOG000253950; - HOVERGEN; HBG000053; - InParanoid; P48059; - OMA; CRECNER; - OrthoDB; EOG091G08K3; - PhylomeDB; P48059; - TreeFam; TF314113; - Reactome; R-HSA-446353; Cell-extracellular matrix interactions Reactome; R-HSA-446388; Regulation of cytoskeletal remodeling and cell spreading by IPP complex components ChiTaRS; LIMS1; human EvolutionaryTrace; P48059; - GeneWiki; LIMS1; - GenomeRNAi; 3987; - PRO; PR:P48059; - Proteomes; UP000005640; Chromosome 2 Bgee; ENSG00000169756; - CleanEx; HS_LIMS1; - ExpressionAtlas; P48059; baseline and differential Genevisible; P48059; HS GO; GO:0005911; C:cell-cell junction; IMP:UniProtKB GO; GO:0005829; C:cytosol; TAS:Reactome GO; GO:0005925; C:focal adhesion; IDA:UniProtKB GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB GO; GO:0007569; P:cell aging; TAS:ProtInc GO; GO:0034329; P:cell junction assembly; TAS:Reactome GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEP:UniProtKB GO; GO:0045184; P:establishment of protein localization; IMP:UniProtKB GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB GO; GO:0010811; P:positive regulation of cell-substrate adhesion; IMP:UniProtKB GO; GO:0051894; P:positive regulation of focal adhesion assembly; IMP:UniProtKB GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB GO; GO:0043547; P:positive regulation of GTPase activity; IMP:UniProtKB GO; GO:1901224; P:positive regulation of NIK/NF-kappaB signaling; IMP:CAFA GO; GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IMP:UniProtKB GO; GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IMP:CAFA InterPro; IPR017351; PINCH InterPro; IPR001781; Znf_LIM PANTHER; PTHR24210; PTHR24210; 1 Pfam; PF00412; LIM; 5 PIRSF; PIRSF038003; PINCH; 1 SMART; SM00132; LIM; 5 PROSITE; PS00478; LIM_DOMAIN_1; 4 PROSITE; PS50023; LIM_DOMAIN_2; 5 1: Evidence at protein level; 3D-structure; Acetylation; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; LIM domain; Membrane; Metal-binding; Reference proteome; Repeat; Zinc INIT_MET 1 1 Removed. CHAIN 2 325 LIM and senescent cell antigen-like- containing domain protein 1 /FTId=PRO_0000075888 DOMAIN 10 62 LIM zinc-binding 1. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 71 121 LIM zinc-binding 2. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 135 184 LIM zinc-binding 3. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 193 243 LIM zinc-binding 4. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 252 303 LIM zinc-binding 5. {ECO:0000255|PROSITE- ProRule:PRU00125} MOD_RES 2 2 N-acetylalanine VAR_SEQ 1 1 M -> MLGVAAGMTHSNM (in isoform 2) /FTId=VSP_042672 VAR_SEQ 1 1 M -> MAFSGRARPCIIPENEEIPRAALNTVHEANGTEDER AVSKLQRRHSDVKVYKEFCDFYAKFNM (in isoform 3). /FTId=VSP_043210 VAR_SEQ 1 1 M -> MTCNM (in isoform 4). /FTId=VSP_043211 VAR_SEQ 1 1 M -> MTALQLKELSHSGLYRRRRDRPDSLRVNGLPEEELS NM (in isoform 5) /FTId=VSP_043212 MUTAGEN 42 42 F->A: Loss of interaction with ILK and loss of localization to focal adhesion MUTAGEN 56 56 R->A: Alters interaction with ILK MUTAGEN 61 61 H->D: Alters interaction with ILK MUTAGEN 62 62 D->A: Alters interaction with ILK MUTAGEN 66 66 L->D: Alters interaction with ILK CONFLICT 78 78 I -> T (in Ref. 5; AAH05341) CONFLICT 262 262 D -> G (in Ref. 5; AAH05341) TURN 11 13 {ECO:0000244|PDB:4HI8} STRAND 22 26 {ECO:0000244|PDB:4HI8} STRAND 29 32 {ECO:0000244|PDB:4HI8} TURN 33 35 {ECO:0000244|PDB:4HI8} TURN 39 41 {ECO:0000244|PDB:4HI8} HELIX 46 48 {ECO:0000244|PDB:4HI8} STRAND 51 53 {ECO:0000244|PDB:4HI8} STRAND 56 58 {ECO:0000244|PDB:4HI8} HELIX 60 66 {ECO:0000244|PDB:4HI8} STRAND 72 74 {ECO:0000244|PDB:2D8X} STRAND 83 85 {ECO:0000244|PDB:2D8X} STRAND 88 90 {ECO:0000244|PDB:2D8X} TURN 92 94 {ECO:0000244|PDB:2D8X} STRAND 98 100 {ECO:0000244|PDB:2D8X} STRAND 105 107 {ECO:0000244|PDB:2D8X} STRAND 110 112 {ECO:0000244|PDB:2D8X} STRAND 115 117 {ECO:0000244|PDB:2D8X} HELIX 119 126 {ECO:0000244|PDB:2D8X} TURN 136 138 {ECO:0000244|PDB:2COR} STRAND 150 152 {ECO:0000244|PDB:2COR} TURN 156 158 {ECO:0000244|PDB:2COR} STRAND 162 164 {ECO:0000244|PDB:2COR} STRAND 173 175 {ECO:0000244|PDB:2COR} STRAND 178 180 {ECO:0000244|PDB:2COR} HELIX 182 186 {ECO:0000244|PDB:2COR} TURN 194 197 {ECO:0000244|PDB:1NYP} STRAND 210 213 {ECO:0000244|PDB:1NYP} TURN 214 216 {ECO:0000244|PDB:1NYP} TURN 220 222 {ECO:0000244|PDB:1NYP} STRAND 227 229 {ECO:0000244|PDB:1NYP} STRAND 232 234 {ECO:0000244|PDB:1NYP} STRAND 237 239 {ECO:0000244|PDB:1NYP} HELIX 241 247 {ECO:0000244|PDB:1NYP} SEQUENCE 325 AA; 37251 MW; E665FEB11D849CAE CRC64; MANALASATC ERCKGGFAPA EKIVNSNGEL YHEQCFVCAQ CFQQFPEGLF YEFEGRKYCE HDFQMLFAPC CHQCGEFIIG RVIKAMNNSW HPECFRCDLC QEVLADIGFV KNAGRHLCRP CHNREKARGL GKYICQKCHA IIDEQPLIFK NDPYHPDHFN CANCGKELTA DARELKGELY CLPCHDKMGV PICGACRRPI EGRVVNAMGK QWHVEHFVCA KCEKPFLGHR HYERKGLAYC ETHYNQLFGD VCFHCNRVIE GDVVSALNKA WCVNCFACST CNTKLTLKNK FVEFDMKPVC KKCYEKFPLE LKKRLKKLAE TLGRK
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References

1. J Biol Chem. 2009 Feb 27;284(9):5836-44. 2. Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20677-82.

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Cat# AO1591a
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