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GCK Antibody

Purified Mouse Monoclonal Antibody

  • E - GCK Antibody AO1593a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - GCK Antibody AO1593a
    Figure 1: Western blot analysis using GCK mAb against human GCK (AA: 1-198) recombinant protein. (Expected MW is 48.2 kDa)
  • WB - GCK Antibody AO1593a
    Figure 2: Western blot analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35557
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G6
Isotype IgG1
Calculated MW 52kDa
Description Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Immunogen Purified recombinant fragment of human GCK expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2645
Other Names Glucokinase,, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGCK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GCK
Function Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
Cellular Location Cytoplasm. Nucleus. Note=Under low glucose concentrations, GCK associates with GKRP and the inactive complex is recruited to the hepatocyte nucleus
Tissue Location Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver
Research Areas
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1. Mol Endocrinol. 2009 Dec;23(12):1983-9. 2. Int J Mol Med. 2009 Aug;24(2):233-46.

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$ 385.00
Cat# AO1593a
(40 western blots)
Availability: 7-10 days
Bulk Size
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