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PSMB8 Antibody

Purified Mouse Monoclonal Antibody

  • E - PSMB8 Antibody AO1605a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - PSMB8 Antibody AO1605a
    Figure 1: Western blot analysis using PSMB8 mAb against human PSMB8 (AA: 1-272) recombinant protein. (Expected MW is 55.2 kDa)
  • WB - PSMB8 Antibody AO1605a
    Figure 2: Western blot analysis using PSMB8 mouse mAb against Hela (1), MCF-7 (2), A431 (3), RAJI (4), MOTL4 (5) and PC-12 (6) cell lysate.
  • IHC - PSMB8 Antibody AO1605a
    Figure 3: Immunohistochemical analysis of paraffin-embedded intima cancer tissues using PSMB8 mouse mAb with DAB staining.
  • IHC - PSMB8 Antibody AO1605a
    Figure 4: Immunohistochemical analysis of paraffin-embedded colon tissues using PSMB8 mouse mAb with DAB staining.
  • IF - PSMB8 Antibody AO1605a
    Figure 5: Immunofluorescence analysis of Hela cells using PSMB8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28062
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 1A5
Isotype IgG1
Calculated MW 30kDa
Description The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Immunogen Purified recombinant fragment of human PSMB8 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 5696
Other Names Proteasome subunit beta type-8,, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein, PSMB8, LMP7, PSMB5i, RING10, Y2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMB8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMB8
Synonyms LMP7, PSMB5i, RING10, Y2
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Cellular Location Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00809}. Nucleus
Research Areas
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1. Genes Chromosomes Cancer. 2009 May;48(5):410-8. 2. Respir Med. 2010 Jun;104(6):889-94. Epub 2010 Feb 11.

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$ 385.00
Cat# AO1605a
(40 western blots)
Availability: 7-10 days
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