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DLK1 Antibody

Purified Mouse Monoclonal Antibody

  • E - DLK1 Antibody AO1630a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - DLK1 Antibody AO1630a
    Figure 1: Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa)
  • IF - DLK1 Antibody AO1630a
    Figure 2: Immunofluorescence analysis of U251 cells using DLK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - DLK1 Antibody AO1630a
    Figure 3: Flow cytometric analysis of NIH/3T3 cells using DLK1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P80370
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A10
Isotype IgG1
Calculated MW 41kDa
Description This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.
Immunogen Purified recombinant fragment of human DLK1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 8788
Other Names Protein delta homolog 1, DLK-1, pG2, Fetal antigen 1, FA1, DLK1, DLK
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDLK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DLK1
Synonyms DLK
Function May have a role in neuroendocrine differentiation.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue
Research Areas
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1. Epigenetics. 2009 Oct 1;4(7):469-75. 2. Mol Biol Cell. 2009 Jul;20(14):3353-62. 

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$ 385.00
Cat# AO1630a
(40 western blots)
Availability: 7-10 days
Bulk Size
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