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IL1B Antibody

Purified Mouse Monoclonal Antibody

     
  • E - IL1B Antibody AO1632a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - IL1B Antibody AO1632a
    Figure 1: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)
  • WB - IL1B Antibody AO1632a
    Figure 2: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - IL1B Antibody AO1632a
    Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.
  • IHC - IL1B Antibody AO1632a
    Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.
  • IF - IL1B Antibody AO1632a
    Figure 5: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P01584
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A6
Isotype IgG1
Calculated MW 31kDa
Description The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Immunogen Purified recombinant fragment of human IL1B expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3553
Other Names Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1B
Synonyms IL1F2
Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Cellular Location Cytoplasm, cytosol. Lysosome. Secreted, exosome {ECO:0000250|UniProtKB:P10749}. Cytoplasmic vesicle, autophagosome. Secreted Note=The precursor is cytosolic. In response to inflammasome- activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B- containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. {ECO:0000250|UniProtKB:P10749, ECO:0000269|PubMed:11728343, ECO:0000269|PubMed:15192144, ECO:0000305|PubMed:24201029}
Tissue Location Expressed in activated monocytes/macrophages (at protein level).
Research Areas
Citations (0)

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References

1. Cell Signal. 2009 Dec;21(12):1935-44.  2. Nat Struct Mol Biol. 2009 Sep;16(9):945-52.

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$ 385.00
Cat# AO1632a
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