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IL1B Antibody

Purified Mouse Monoclonal Antibody

  • E - IL1B Antibody AO1632a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - IL1B Antibody AO1632a
    Figure 1: Western blot analysis using IL1B mAb against human IL1B (AA: 126-261) recombinant protein. (Expected MW is 41 kDa)
  • WB - IL1B Antibody AO1632a
    Figure 2: Western blot analysis using IL1B mAb against HEK293 (1) and IL1B-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - IL1B Antibody AO1632a
    Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using IL1B mouse mAb with DAB staining.
  • IHC - IL1B Antibody AO1632a
    Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using IL1B mouse mAb with DAB staining.
  • IF - IL1B Antibody AO1632a
    Figure 5: Immunofluorescence analysis of HepG2 cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01584
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A6
Isotype IgG1
Calculated MW 31kDa
Description The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Immunogen Purified recombinant fragment of human IL1B expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3553
Other Names Interleukin-1 beta, IL-1 beta, Catabolin, IL1B, IL1F2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1B
Synonyms IL1F2
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Cellular Location Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins
Research Areas
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1. Cell Signal. 2009 Dec;21(12):1935-44.  2. Nat Struct Mol Biol. 2009 Sep;16(9):945-52.

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$ 385.00
Cat# AO1632a
(40 western blots)
Availability: 7-10 days
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