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NEDD8 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - NEDD8 Antibody AO1665a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NEDD8 Antibody AO1665a

    Figure 1: Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
  • WB - NEDD8 Antibody AO1665a
    Figure 2: Western blot analysis using NEDD8 mouse mAb against C6 (1) and Hela (2) cell lysate.
  • IHC - NEDD8 Antibody AO1665a
    Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 mouse mAb with DAB staining.
  • IF - NEDD8 Antibody AO1665a
    Figure 4: Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - NEDD8 Antibody AO1665a
    Figure 5: Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
  • SPECIFICATION
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q15843
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B8
Isotype IgG1
Calculated MW 9kDa
Description Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Immunogen Purified recombinant fragment of human NEDD8 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4738
Other Names NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEDD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD8
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Cellular Location Nucleus. Note=Mainly nuclear
Tissue Location Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes
Research Areas
Citations (0)

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References

1. Cell. 2009 Jul 23;138(2):389-403. 2. Biochem Biophys Res Commun. 2009 Apr 10;381(3):443-7.

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$ 385.00
Cat# AO1665a
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