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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   INCENP Antibody   

INCENP Antibody

Purified Mouse Monoclonal Antibody

  • E - INCENP Antibody AO1738a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - INCENP Antibody AO1738a
    Figure 1: Western blot analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)
  • WB - INCENP Antibody AO1738a
    Figure 2: Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - INCENP Antibody AO1738a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using INCENP mouse mAb with DAB staining.
  • IHC - INCENP Antibody AO1738a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.
  • IF - INCENP Antibody AO1738a
    Figure 5: Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - INCENP Antibody AO1738a
    Figure 6: Flow cytometric analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NQS7
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3D2
Isotype IgG1
Calculated MW 105.4kDa
Description In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis
Immunogen Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3619
Other Names Inner centromere protein, INCENP
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsINCENP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
Cellular Location Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes
Research Areas
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1.J Cell Biol. 2009 Nov 30;187(5):637-53. 2.Genes Cells. 2011 Jun;16(6):652-69.

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$ 385.00
Cat# AO1738a
(40 western blots)
Availability: 7-10 days
Bulk Size
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