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NR6A1 Antibody

Purified Mouse Monoclonal Antibody

  • E - NR6A1 Antibody AO1742a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NR6A1 Antibody AO1742a
    Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa)
  • WB - NR6A1 Antibody AO1742a
    Figure 2: Western blot analysis using NR6A1 mAb against HEK293 (1) and NR6A1 (AA: 65-118)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - NR6A1 Antibody AO1742a
    Figure 3: Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.
  • IF - NR6A1 Antibody AO1742a
    Figure 4: Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - NR6A1 Antibody AO1742a
    Figure 5: Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).
  • IHC - NR6A1 Antibody AO1742a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.
  • IHC - NR6A1 Antibody AO1742a
    Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15406
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G8
Isotype IgG1
Calculated MW 54.3kDa
Description This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. The gene expresses at least alternatively spliced transcript variants.
Immunogen Purified recombinant fragment of human NR6A1 (AA: 65-118) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2649
Other Names Nuclear receptor subfamily 6 group A member 1, Germ cell nuclear factor, GCNF, hGCNF, Retinoid receptor-related testis-specific receptor, RTR, hRTR, NR6A1, GCNF
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNR6A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR6A1
Synonyms GCNF
Function Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis (By similarity).
Cellular Location Nucleus.
Tissue Location Shows highest expression in the germ cells of the adult testis.
Research Areas
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1.Biochem Biophys Res Commun. 2010 Dec 10;403(2):178-83.2.Cell. 2005 Sep 23;122(6):957-68.

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$ 385.00
Cat# AO1742a
(40 western blots)
Availability: 7-10 days
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