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ENO2 Antibody

Purified Mouse Monoclonal Antibody

  • E - ENO2 Antibody AO1744a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ENO2 Antibody AO1744a
    Figure 1: Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)
  • WB - ENO2 Antibody AO1744a
    Figure 2: Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
  • FC - ENO2 Antibody AO1744a
    Figure 3: Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).
  • IHC - ENO2 Antibody AO1744a
    Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.
  • IHC - ENO2 Antibody AO1744a
    Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09104
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 5D3
Isotype IgG1
Calculated MW 47.3kDa
Description This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Immunogen Purified recombinant fragment of human ENO2 (AA: 251-433) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2026
Other Names Gamma-enolase,, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENO2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ENO2
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Cellular Location Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
Research Areas
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1.Neurology. 2011 Aug 16;77(7):623-30. 2.Lung Cancer. 2011 Feb;71(2):224-8.

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$ 385.00
Cat# AO1744a
(40 western blots)
Availability: 7-10 days
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