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POU3F2 Antibody

Purified Mouse Monoclonal Antibody

  • E - POU3F2 Antibody AO1751a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - POU3F2 Antibody AO1751a
    Figure 1: Western blot analysis using POU3F2 mAb against human POU3F2 recombinant protein. (Expected MW is 42.1 kDa)
  • WB - POU3F2 Antibody AO1751a
    Figure 2: Western blot analysis using POU3F2 mouse mAb against HeLa (1), and SK-N-SH (2) cell lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20265
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6H12
Isotype IgG1
Calculated MW 46.9kDa
Description This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells.
Immunogen Purified recombinant fragment of human POU3F2 (AA: 214-362) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5454
Other Names POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF-7, POU3F2, BRN2, OCT7, OTF7
Dilution E~~1/10000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU3F2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU3F2
Synonyms BRN2, OCT7, OTF7
Function Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed specifically in the neuroectodermal cell lineage
Research Areas
Citations (0)

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1.Cancer Res. 2008 Oct 1;68(19):7788-94.2.Mol Cancer. 2006 Dec 12;5:72.

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$ 385.00
Cat# AO1751a
(40 western blots)
Availability: 7-10 days
Bulk Size
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