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SPIB Antibody

Purified Mouse Monoclonal Antibody

  • E - SPIB Antibody AO1754a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SPIB Antibody AO1754a
    Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
  • WB - SPIB Antibody AO1754a
    Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
  • IF - SPIB Antibody AO1754a
    Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - SPIB Antibody AO1754a
    Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
  • IHC - SPIB Antibody AO1754a
    Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
  • IHC - SPIB Antibody AO1754a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01892
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 4G5
Isotype IgG1
Calculated MW 28.8kDa
Description The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 6689
Other Names Transcription factor Spi-B, SPIB
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPIB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
Cellular Location Isoform 1: Nucleus.
Tissue Location Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver
Citations (0)

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1.J Gen Virol. 2010 Dec;91(Pt 12):3042-52.2.Eur J Immunol. 2008 Sep;38(9):2389-400.

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$ 385.00
Cat# AO1754a
(40 western blots)
Availability: 7-10 days
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