|Application ||WB, IHC, FC, ICC, E|
|Description||The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene.|
|Immunogen||Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Transcription factor Spi-B, SPIB|
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SPIB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.|
|Cellular Location||Isoform 1: Nucleus|
|Tissue Location||Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver|
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Provided below are standard protocols that you may find useful for product applications.
1.J Gen Virol. 2010 Dec;91(Pt 12):3042-52.2.Eur J Immunol. 2008 Sep;38(9):2389-400.
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