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UTF1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - UTF1 Antibody AO1767a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - UTF1 Antibody AO1767a
    Figure 1: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)
    detail
  • WB - UTF1 Antibody AO1767a
    Figure 2: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - UTF1 Antibody AO1767a
    Figure 3: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q5T230
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B6
Isotype IgG1
Calculated MW 36.4kDa
Description Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.
Immunogen Purified recombinant fragment of human UTF1 (AA: 148-214) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 8433
Other Names Undifferentiated embryonic cell transcription factor 1, UTF1 (HGNC:12634)
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUTF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UTF1 (HGNC:12634)
Function Acts as a transcriptional coactivator of ATF2.
Cellular Location Nucleus.
Research Areas
Citations (0)
citation

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References

1.Int J Exp Pathol. 2011 Oct;92(5):326-32.2.Stem Cell Res. 2009 May;2(3):211-8.

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$ 385.00
Cat# AO1767a
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