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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NEFH Antibody   

NEFH Antibody

Purified Mouse Monoclonal Antibody

  • E - NEFH Antibody AO1768a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NEFH Antibody AO1768a
    Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa)
  • WB - NEFH Antibody AO1768a
    Figure 2: Western blot analysis using NEFH mAb against HEK293 (1) and NEFH (AA: 968-1020)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - NEFH Antibody AO1768a
    Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using NEFH mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12036
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8H10
Isotype IgG1
Calculated MW 112.4kDa
Description Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Immunogen Purified recombinant fragment of human NEFH (AA: 968-1020) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4744
Other Names Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA0845, NFH
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEFH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0845, NFH
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Research Areas
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1.J Neurol Sci. 2011 May 15;304(1-2):117-21. 2.Neurochem Res. 2011 Dec;36(12):2287-91.

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$ 385.00
Cat# AO1768a
(40 western blots)
Availability: 7-10 days
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