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ABCB5 Antibody

Purified Mouse Monoclonal Antibody

  • E - ABCB5 Antibody AO1779a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ABCB5 Antibody AO1779a
    Figure 1: Western blot analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)
  • WB - ABCB5 Antibody AO1779a
    Figure 2: Western blot analysis using ABCB5 mouse mAb against A431 (1) cell lysate.
  • IF - ABCB5 Antibody AO1779a
    Figure 3: Immunofluorescence analysis of HepG2 cells using ABCB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - ABCB5 Antibody AO1779a
    Figure 4: Flow cytometric analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).
  • IHC - ABCB5 Antibody AO1779a
    Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.
  • IHC - ABCB5 Antibody AO1779a
    Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q2M3G0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5H3C6
Isotype IgG1
Calculated MW 89.8kDa
Description ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules
Immunogen Purified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 340273
Other Names ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB5
Function Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:12960149}
Tissue Location Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.
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1.Gastroenterology. 2011 Jan;140(1):344-55. 2.Cancer Res. 2011 Aug 1;71(15):5307-16.

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$ 385.00
Cat# AO1779a
(40 western blots)
Availability: 7-10 days
Bulk Size
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