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SPP1 Antibody

Purified Mouse Monoclonal Antibody

  • E - SPP1 Antibody AO1790a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SPP1 Antibody AO1790a
    Figure 1: Western blot analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
  • WB - SPP1 Antibody AO1790a
    Figure 2: Western blot analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - SPP1 Antibody AO1790a
    Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using SPP1 mouse mAb with DAB staining.
  • IHC - SPP1 Antibody AO1790a
    Figure 4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SPP1 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10451
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7C5H12
Isotype IgG1
Calculated MW 35.4kDa
Description The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human SPP1 (AA: 167-314) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6696
Other Names Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPP1
Synonyms BNSP, OPN
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Cellular Location Secreted.
Tissue Location Bone. Found in plasma.
Research Areas
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This protein encoded by this gene belongs to the WD repeat-containing family of proteins, which function in the formation of protein-protein complexes in a variety of biological pathways. This family member appears to function in the determination of mean platelet volume (MPV), and polymorphisms in this gene have been associated with variance in MPV. Alternative splicing of this gene results in multiple transcript variants. ;


1.Blood. 2012 May 31;119(22):5215-20. 2.J Cancer Res Ther. 2011 Apr-Jun;7(2):138-42.

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$ 385.00
Cat# AO1790a
(40 western blots)
Availability: 7-10 days
Bulk Size
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