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CSPG4 Antibody

Purified Mouse Monoclonal Antibody

  • E - CSPG4 Antibody AO1802a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CSPG4 Antibody AO1802a
    Figure 1: Western blot analysis using CSPG4 mAb against human CSPG4 recombinant protein. (Expected MW is 32.5 kDa)
  • WB - CSPG4 Antibody AO1802a
    Figure 2: Western blot analysis using CSPG4 mAb against HEK293 (1) and CSPG4 (AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - CSPG4 Antibody AO1802a
    Figure 3: Flow cytometric analysis of HEK293 cells using CSPG4 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6UVK1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7G4E5
Isotype IgG1
Calculated MW 250.0kDa
Description A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Immunogen Purified recombinant fragment of human CSPG4 (AA: 2247-2308) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1464
Other Names Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan, CSPG4, MCSP
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCSPG4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CSPG4
Synonyms MCSP
Function Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Cellular Location Apical cell membrane; Single- pass type I membrane protein; Extracellular side. Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers Localizes to the plasma membrane of oligodendrocytes (By similarity).
Tissue Location Detected only in malignant melanoma cells.
Research Areas
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This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.


1.Cancer Res. 2011 Dec 15;71(24):7410-22. 2.Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57.

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$ 385.00
Cat# AO1802a
(40 western blots)
Availability: 7-10 days
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