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TWIST1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - TWIST1 Antibody AO1809a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TWIST1 Antibody AO1809a
    Figure 1: Western blot analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)
  • WB - TWIST1 Antibody AO1809a
    Figure 2: Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - TWIST1 Antibody AO1809a
    Figure 3: Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
  • IF - TWIST1 Antibody AO1809a
    Figure 4: Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - TWIST1 Antibody AO1809a
    Figure 5: Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
  • IHC - TWIST1 Antibody AO1809a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
  • IHC - TWIST1 Antibody AO1809a
    Figure 7: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q15672
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 10E4E6
Isotype IgG1
Calculated MW 21kDa
Description Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Immunogen Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 7291
Other Names Twist-related protein 1, Class A basic helix-loop-helix protein 38, bHLHa38, H-twist, TWIST1, BHLHA38, TWIST
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTWIST1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TWIST1
Synonyms BHLHA38, TWIST
Function Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
Cellular Location Nucleus.
Tissue Location Subset of mesodermal cells.
Research Areas
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Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. ; ; ;

References

1. Cancer Res. 2013 Jan 15;73(2):662-71. 2. Cancer Res. 2012 Dec 15;72(24):6382-92.

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$ 385.00
Cat# AO1809a
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