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ZFP42 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ZFP42 Antibody AO1813a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ZFP42 Antibody AO1813a
    Figure 1: Western blot analysis using ZFP42 mAb against human ZFP42 recombinant protein. (Expected MW is 32.7 kDa)
  • WB - ZFP42 Antibody AO1813a
    Figure 2: Western blot analysis using ZFP42 mAb against HEK293 (1) and ZFP42 (AA: 249-310)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - ZFP42 Antibody AO1813a
    Figure 3: Western blot analysis using ZFP42 mouse mAb against Jurkat (1), HEK293 (2), Raji (3) and PC-3 (4) cell lysate.
  • FC - ZFP42 Antibody AO1813a
    Figure 4: Flow cytometric analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).
  • IHC - ZFP42 Antibody AO1813a
    Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZFP42 mouse mAb with DAB staining.
  • IHC - ZFP42 Antibody AO1813a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using ZFP42 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession Q96MM3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5E11E7
Isotype IgG1
Calculated MW 34.8kDa
Description ZFP42 involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter.
Immunogen Purified recombinant fragment of human ZFP42 (AA: 249-310) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 132625
Other Names Zinc finger protein 42 homolog, Zfp-42, Reduced expression protein 1, REX-1, hREX-1, Zinc finger protein 754, ZFP42, REX1, ZNF754
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZFP42 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZFP42
Synonyms REX1, ZNF754
Function Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines
Research Areas
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Background

ZFP42 involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter.

References

1. Stem Cell Res. 2011 Jul;7(1):1-16. 2. J Cell Physiol. 2010 Jul;224(1):17-27.

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$ 385.00
Cat# AO1813a
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