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CFLAR Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CFLAR Antibody AO1814a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CFLAR Antibody AO1814a
    Figure 1: Western blot analysis using CFLAR mAb against human CFLAR recombinant protein. (Expected MW is 42.9 kDa)
  • WB - CFLAR Antibody AO1814a
    Figure 2: Western blot analysis using CFLAR mouse mAb against JURKAT (1), 3T3L1 (2) and RAJI (3) cell lysate.
  • FC - CFLAR Antibody AO1814a
    Figure 3: Flow cytometric analysis of JURKAT cells using CFLAR mouse mAb (green) and negative control (red).
  • IHC - CFLAR Antibody AO1814a
    Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CFLAR mouse mAb with DAB staining.
  • IHC - CFLAR Antibody AO1814a
    Figure 5: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using CFLAR mouse mAb with DAB staining.
  • SPECIFICATION
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession O15519
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 7A3D12
Isotype IgG1
Calculated MW 55kDa
Description The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.
Immunogen Purified recombinant fragment of human CFLAR (AA: 100-251) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 8837
Other Names CASP8 and FADD-like apoptosis regulator, Caspase homolog, CASH, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, CLARP, Cellular FLICE-like inhibitory protein, c-FLIP, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, CASP8 and FADD-like apoptosis regulator subunit p43, CASP8 and FADD-like apoptosis regulator subunit p12, CFLAR, CASH, CASP8AP1, CLARP, MRIT
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCFLAR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CFLAR
Synonyms CASH, CASP8AP1, CLARP, MRIT
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue Location Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle
Research Areas
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Background

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. ; ; ;

References

1. PLoS One. 2012;7(9):e44917. 2. Cancer Prev Res (Phila). 2012 Apr;5(4):612-20.

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$ 385.00
Cat# AO1814a
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