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IL2RA Antibody

Purified Mouse Monoclonal Antibody

     
  • E - IL2RA Antibody AO1825a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - IL2RA Antibody AO1825a
    Figure 1: Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
  • WB - IL2RA Antibody AO1825a
    Figure 2: Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - IL2RA Antibody AO1825a
    Figure 3: Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • WB - IL2RA Antibody AO1825a
    Figure 3: Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
  • IHC - IL2RA Antibody AO1825a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining.
  • IHC - IL2RA Antibody AO1825a
    Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P01589
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 1B5D12
Isotype IgG1
Calculated MW 30.8kDa
Description The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Immunogen Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3559
Other Names Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL2RA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL2RA
Function Receptor for interleukin-2.
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. ; ; ; ;

References

1. Ann Hematol. 2012 Oct;91(10):1597-602. 2. Transplant Proc. 2012 May;44(4):1139-42.

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$ 385.00
Cat# AO1825a
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