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ALCAM Antibody

Purified Mouse Monoclonal Antibody

  • E - ALCAM Antibody AO1831a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ALCAM Antibody AO1831a
    Figure 1: Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)
  • WB - ALCAM Antibody AO1831a
    Figure 2: Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - ALCAM Antibody AO1831a
    Figure 3: Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.
  • FC - ALCAM Antibody AO1831a
    Figure 4: Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).
  • IHC - ALCAM Antibody AO1831a
    Figure 5: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using ALCAM mouse mAb with DAB staining.
  • IHC - ALCAM Antibody AO1831a
    Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13740
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 4H9A5
Isotype IgG2b
Calculated MW 65.1kDa
Description This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen Purified recombinant fragment of human ALCAM (AA: 48-216) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 214
Other Names CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MEMD
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
Research Areas
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This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene.  ;


1. Vascul Pharmacol. 2011 Mar-Jun;54(3-6):93-9. 2. Int J Gynecol Cancer. 2011 Apr;21(3):523-8.

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$ 385.00
Cat# AO1831a
(40 western blots)
Availability: 7-10 days
Bulk Size
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