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CD14 Antibody

Purified Mouse Monoclonal Antibody

  • E - CD14 Antibody AO1886a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CD14 Antibody AO1886a
    Figure 1: Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (Expected MW is 46.8 kDa)
  • WB - CD14 Antibody AO1886a
    Figure 2: Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - CD14 Antibody AO1886a
    Figure 3: Immunofluorescence analysis of HepG2 cells using CD14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
  • IHC - CD14 Antibody AO1886a
    Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD14 mouse mAb with DAB staining.
  • IHC - CD14 Antibody AO1886a
    Figure 5: Immunohistochemical analysis of paraffin-embedded colon tissues using CD14 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08571
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H5D8
Isotype IgG2a
Calculated MW 40kDa
Description The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
Immunogen Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 929
Other Names Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD14
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages
Research Areas
Citations (0)

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The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. ;


1. J Immunol. 2012 Dec 15;189(12):5729-44. 2. Iran J Immunol. 2011 Jun;8(2):111-9.

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$ 385.00
Cat# AO1886a
(40 western blots)
Availability: 7-10 days
Bulk Size
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