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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   EIF2A Antibody   

EIF2A Antibody

Purified Mouse Monoclonal Antibody

  • E - EIF2A Antibody AO1904a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EIF2A Antibody AO1904a
    Figure 1: Western blot analysis using EIF2A mAb against human EIF2A (AA: 448-576) recombinant protein. (Expected MW is 40.3 kDa)
  • WB - EIF2A Antibody AO1904a
    Figure 2: Western blot analysis using EIF2A mAb against HEK293 (1) and EIF2A (AA: 448-576)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - EIF2A Antibody AO1904a
    Figure 3: Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.
  • IF - EIF2A Antibody AO1904a
    Figure 4: Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
  • FC - EIF2A Antibody AO1904a
    Figure 5: Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).
  • IHC - EIF2A Antibody AO1904a
    Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2A mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BY44
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A7A8
Isotype IgG1
Calculated MW 65kDa
Description EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes
Immunogen Purified recombinant fragment of human EIF2A (AA: 448-576) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 83939
Other Names Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A, Eukaryotic translation initiation factor 2A, N-terminally processed, EIF2A
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF2A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2A
Function Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.
Tissue Location Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta
Research Areas
Citations (0)

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This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. ; ; ;


1. Mol Biol (Mosk). 2010 Sep-Oct;44(5):859-66. 2. Cancer Res. 2009 Feb 15;69(4):1545-52.

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$ 385.00
Cat# AO1904a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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