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BAD Antibody

Purified Mouse Monoclonal Antibody

  • E - BAD Antibody AO1933a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - BAD Antibody AO1933a
    Figure 1: Western blot analysis using BAD mAb against human BAD (AA: FULL(1-168)) recombinant protein. (Expected MW is 44.3 kDa)
  • WB - BAD Antibody AO1933a
    Figure 2: Western blot analysis using BAD mAb against HEK293 (1) and BAD (AA: FULL(1-168))-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - BAD Antibody AO1933a
    Figure 3: Western blot analysis using BAD mouse mAb against MCF-7 (1), HEK293 (2) cell lysate.
  • FC - BAD Antibody AO1933a
    Figure 4: Flow cytometric analysis of MCF-7 cells using BAD mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92934
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1G5B3
Isotype IgG1
Calculated MW 18.4kDa
Description The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Immunogen Purified recombinant fragment of human BAD (AA: FULL(1-168)) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 572
Other Names Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, BAD, BBC6, BCL2L8
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBAD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BAD
Synonyms BBC6, BCL2L8
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Cellular Location Mitochondrion outer membrane. Cytoplasm {ECO:0000250|UniProtKB:Q61337}. Note=Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm. {ECO:0000250|UniProtKB:Q61337}
Tissue Location Expressed in a wide variety of tissues.
Research Areas
Citations (0)

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Chromobox homolog 8 (CBX8), a Polycomb Group protein that interacts with MLL-AF9 and TIP60, plays an essential role in MLL-AF9 transcriptional regulation and leukemogenesis. CBX8, which is part of one of the PRC1 complexes, regulates proliferation of diploid human and mouse fibroblasts through direct binding to the INK4A-ARF locus. ; ;


1. Clin Cancer Res. 2011 Oct 1;17(19):6356-66.2. Mol Cancer Res. 2010 Aug;8(8):1116-25.

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$ 325.00
Cat# AO1933a
(40 western blots)
Availability: 7-10 days
Bulk Size
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