|Application ||WB, IHC, FC, ICC, E|
|Description||The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.|
|Immunogen||Synthesized peptide fragment of human SLINGSHOT-1L (AA: 1032-1044 ) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Protein phosphatase Slingshot homolog 1, 126.96.36.199, 188.8.131.52, SSH-like protein 1, SSH-1L, hSSH-1L, SSH1, KIAA1298, SSH1L|
FC~~1/200 - 1/400
IHC~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SLINGSHOT-1L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cleavage furrow. Midbody. Note=Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion Also localized to the cleavage furrow and the midbody during cytokinesis|
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