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EIF5A Antibody

Purified Mouse Monoclonal Antibody

  • E - EIF5A Antibody AO2010a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - EIF5A Antibody AO2010a
    Figure 1: Western blot analysis using EIF5A mAb against human EIF5A(AA: 1-154) recombinant protein. (Expected MW is 42.8 kDa)
  • WB - EIF5A Antibody AO2010a
    Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: 1-154)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - EIF5A Antibody AO2010a
    Figure 3: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).
  • IHC - EIF5A Antibody AO2010a
    Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P63241
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4E10F6
Isotype IgG1
Calculated MW 16.8kDa
Description EIF5A (eukaryotic translation initiation factor 5A) is a protein-coding gene. Diseases associated with EIF5A include lung adenocarcinoma, and intrahepatic cholangiocarcinoma, and among its related super-pathways are Post-translational protein modification and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include ribosome binding and RNA binding. An important paralog of this gene is EIF5AL1.
Immunogen Purified recombinant fragment of human EIF5A (AA:1-154) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1984
Other Names Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF5A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF5A
Function mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Cellular Location Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus, nuclear pore complex. Note=Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions
Tissue Location Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level)
Citations (0)

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Int J Cancer. 2011 Jul 1;129(1):143-50.Int J Cancer. 2010 Aug 15;127(4):968-76.

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$ 385.00
Cat# AO2010a
(40 western blots)
Availability: 7-10 days
Bulk Size
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