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NOX4 Antibody

Purified Mouse Monoclonal Antibody

  • E - NOX4 Antibody AO2017a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NOX4 Antibody AO2017a

    Figure 2:Western blot analysis using NOX4 mAb against human NOX4 (AA: 210-310) recombinant protein. (Expected MW is 25.3 kDa)

  • WB - NOX4 Antibody AO2017a

    Figure 3:Western blot analysis using NOX4 mAb against HEK293 (1) and NOX4 (AA: 210-310)-hIgGFc transfected HEK293 (2) cell lysate.

  • IF - NOX4 Antibody AO2017a

    Figure 4:Immunofluorescence analysis of Hela cells using NOX4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - NOX4 Antibody AO2017a

    Figure 5:Flow cytometric analysis of Hela cells using NOX4 mouse mAb (green) and negative control (red).

  • IHC - NOX4 Antibody AO2017a

    Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NOX4 mouse mAb with DAB staining.

  • IHC - NOX4 Antibody AO2017a

    Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NOX4 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NPH5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3H2G11
Isotype IgG1
Calculated MW 67kDa
Description This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Immunogen Purified recombinant fragment of human NOX4 (AA: 210-310) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 50507
Other Names NADPH oxidase 4, 1.6.3.-, Kidney oxidase-1, KOX-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, NOX4, RENOX
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOX4
Synonyms RENOX
Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus
Tissue Location Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells
Research Areas
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Stem Cells Dev. 2012 Aug 10;21(12):2212-21.Cancer Biol Ther. 2010 Aug 1;10(3):223-31.

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$ 385.00
Cat# AO2017a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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