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TUBB1 Antibody

Purified Mouse Monoclonal Antibody

  • E - TUBB1 Antibody AO2025a

    Figure 1:Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TUBB1 Antibody AO2025a

    Figure 2:Western blot analysis using TUBB1 mAb against human TUBB1 (AA: 33-166) recombinant protein. (Expected MW is 40.5 kDa)

  • WB - TUBB1 Antibody AO2025a

    Figure 3:Western blot analysis using TUBB1 mAb against HEK293 (1) and TUBB1 (AA: 33-166)-hIgGFc transfected HEK293 (2) cell lysate.

  • WB - TUBB1 Antibody AO2025a

    Figure 4:Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate.

  • IF - TUBB1 Antibody AO2025a

    Figure 5:Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • FC - TUBB1 Antibody AO2025a

    Figure 6:Flow cytometric analysis of A431 cells using TUBB1 mouse mAb (green) and negative control (red).

  • IHC - TUBB1 Antibody AO2025a

    Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.

  • IHC - TUBB1 Antibody AO2025a

    Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H4B7
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2A1A9
Isotype IgG1
Calculated MW 50.3kDa
Description This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
Immunogen Purified recombinant fragment of human TUBB1 (AA: 33-166) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 81027
Other Names Tubulin beta-1 chain, TUBB1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB1
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins
Research Areas
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Cancer Res. 2012 Sep 15;72(18):4744-52.Cytoskeleton (Hoboken). 2011 Mar;68(3):175-87.

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$ 385.00
Cat# AO2025a
(40 western blots)
Availability: 7-10 days
Bulk Size
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